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Laboratory Course [1]

Paper Code: 
BCH 103
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

1.  Introduction to concentration scales- Definition , units of various concentration terms.

2.  Preparation of solutions and their standardization.

3.  Preparation of standard buffers and determination of pH of a solution.

4.  Verification of Beer Lambert’s Law.

5.  Qualitative tests for :

  • Carbohydrates
  • Proteins and amino acids
  • Lipids

6.  Determination of saponification value and iodine number of fats.

7.  Estimation of ascorbic acid by titrimetric method using 2,6-dichlorophenol indophenol.

8.  Estimation of

  • Carbohydrate by anthrone method/Benedicts method.
  • Blood glucose by the methods

                   a) Folin-Wu

                   b) Nelson-Somogyi

9.  Isolation and assay of glycogen from rat liver.

10. Extraction of total lipids by Folch method
 

Unit I: 
References: 
  1. Vogel’s Textbook of Quantitative Chemical Analysis, Fifth Edition, G.H. Jeffery, J. Bassett. J. Mendham, R.C. Denney, Longman Scientific and Technical Publication, England, 1991.
  2. An Introduction to Practical Biochemistry, Third edition, Plummer, Tata Mc Graw Hill, 2002.

 

Academic Year: 
2018-2019 [2]

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Source URL: https://my-campus.iisuniv.ac.in/courses/subjects/laboratory-course-7

Links:
[1] https://my-campus.iisuniv.ac.in/courses/subjects/laboratory-course-7
[2] https://my-campus.iisuniv.ac.in/academic-year/2018-2019