Course Objectives :
The course aims to endow students with the knowledge of the structure-function relationship of biomolecules, and their importance with regard to maintenance and perpetuation of the living systems.
Course Outcomes (Cos):
|
COURSE |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
|
|---|---|---|---|---|
|
Paper Code |
Paper Title |
|||
|
CHY 425(B) |
Biomolecules and Bio-organic Chemistry |
The students will be able to-
CO148-demonstrate remarkable properties and functions of enzymes and illustrate mechanisms of enzyme catalyzed reactions. CO149-describe major pathways of carbohydrate metabolism. CO150-recognize the structures of amino acids and elucidate common pathways of amino acid catabolism. CO151-summarize the functions of proteins and identify the influence of the three-dimensional shape and subunits of a protein on its function. CO152-analyse the role of fat in energy production, membrane synthesis, and production of bioactive molecules and describe the structure, biosynthesis, oxidation and storage of fatty acids. CO153-learn the structure and functions of RNA and DNA. CO154-correlate how the biomolecules such as proteins, carbohydrates, lipids, nucleic acids are made from the simple precursors. CO155-interpret the structure-function relationships of the proteins, carbohydrates, lipids, and nucleic acids. |
Class lectures
Tutorials
Group discussions
Question preparation: Subjective type- Long answer Short answer Objective type- Multiple choice questions One answer/two answer type questions Assertion and reasoning |
PowerPoint Presentation
Written test
Google Quiz
Assignment
Semester End Exam |
Remarkable properties of enzymes like catalytic power, specificity and regulation, nomenclature and classification. Fischer's lock and key and Koshland's induced fit hypothesis.Enzyme kinetics: Michaelis-Menten and Lineweaver-Burk plots, Bisubstrate reactions. Enzyme inhibition. Factors affecting enzyme catalysis.Examples of some typical enzyme mechanisms for Chymotrypsin, Ribonuclease, Lysozyme, & Carboxipeptidase A.
Affinity labeling and enzyme modification by site-directed mutagenesis.
Structure & classification of carbohydrates. Glycolysis, fate of pyruvate under anaerobic conditions, citric acid cycle, oxidative phosphorylation (electron transport system), gluconeogenesis and glucogenolysis, C4 pathway, pentose phosphate pathway and photosynthesis: C3, C4 & CAM pathway
Classification of amino acids. Degradation of amino acids (C3, C4, C5 family), urea cycle, uric acid and ammonia formation. Proteins (Structure and Functions): Primary, secondary, tertiary and quaternary structure. Extraction and purification techniques.
Classification and nomenclature of Fatty acids. Classification, Structure and functions of lipids. Biosynthesis of saturated and unsaturated fatty acids, ketone bodies, membrane lipids-cholesterol, phospholipid and glycolipid, biosynthesis of fat soluble vitamins, biosynthesis of Eicosonoids (prostaglandin, leucotriens and thromboxane). Metabolism of lipid and fat bodies: Beta-oxidation and channelling of the products to ATP production: Minor pathway of fatty acid oxidation: Alpha and omega oxidation.
Chemical and enzymatic hydrolysis, structure and functions of DNA, RNA (m-RNA, t-RNA, r-RNA), an overview of gene expression (replication, transcription and translation), genetic code (origin, Wobble hypothesis and other important features).
Links:
[1] https://my-campus.iisuniv.ac.in/courses/subjects/biomolecules-and-bio-organic-chemistry-6
[2] https://my-campus.iisuniv.ac.in/academic-year/2020-2021-0